An innovative FastDigest™ formulation of Fermentas restriction enzymes for target DNA digestion in only 5 minutes. Visit for updated list of. I am trying to study a promoter using pCAMBIA plasmid, therefore I need to cut the 35S promoter and replace it with my promoter. However, after several. Bgl II generates ends that are compatible with fragments generated by BamH I, Bcl I, Nde II, Sau 3A and Xho II. Subsequent re-cutting with Bgl II yields >95% of the typical pattern of?DNA × Bgl II fragments. Heat inactivation: No inactivation of Bgl II after incubation at 65 °C Missing: fermentas ‎| ‎Must include: ‎fermentas.


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After running your rxn on a gel, you should be able to tell if it was successful.

Restrictase Bgl II | Practical Molecular Biology

To be thorough, you can run controls of single enzyme digests and undigested. Don't bother cleaning up your DNA between reactions because you will lose too much and it is unnecessary.

The most important ingredient in restriction enzyme buffers is NaCl. As long as the final NaCl concentration is correct your particular restriction enzyme it will cut bglii fermentas.

Double digestion with BamHI and BglII - Molecular Cloning

In recent years, for making different competitive IPCs, several strategies have been used including altering size of target DNA by insertion, deletion, substitution or placing a new restriction site in the target DNA which is not present in the wild DNA type.

Using the last strategy would add an extra step to the detection because following the PCR; the PCR product should be subjected to enzyme digestion. Occasionally, false negative results may occur due to fail of enzymatic digestions 22 - They made a DNA fragment containing a primer annealing site for target gene with the heterologous region at the middle derived from the pGEM plasmid One of the limitations for using the above strategies for producing IPC is that various steps and genetic engineering methods were applied, making the process difficult, complicated, time-consuming and costly whereas in the present study by a simple, easy and cost-effective strategy, an IPC fragment was developed.

The bglii fermentas showed that higher concentration of the IPC plasmid would prevent amplification of the target gene. In the current study, concerning the fact that IPC was longer than target gene in size, advantage in detection was seen because, in amplification competition between target gene and IPC, target gene is always successful.

The results of this study concluded that PCR amplification of overlapping initial and ending parts of a diagnostic gene, pasting the fragments and eventually cloning is a simple and cost-effective strategy to make longer target gene and a competitive IPC.

This IPC could be routinely used for improved detection of target genes. Acknowledgements The authors would like to acknowledge and appreciate the faculty of medicine and AJA University of Medical Sciences for their support and contribution to this study.


There is no financial interest. Measurement of the antibiotic susceptibility of Coxiella burnetii using real time PCR. Int J Antimicrob Agents.


Real-time PCR in clinical microbiology: Construction and evaluation of a microbiological positive process internal control for PCR-based examination of food samples for Listeria monocytogenes bglii fermentas Salmonella enterica.

Int J Food Microbiol. Polymerase chain reaction decontamination: Primary visual cortical neurons were prepared as described bglii fermentas 9. Briefly, 1—2-day-old Sprague-Dawley rat pups were anesthetized with CO2 and killed by decapitation.

FastDigest BglII

bglii fermentas The brains were removed and cleansed of meninges and surface blood vessels, and the bglii fermentas were dissected, trypsinized, and triturated to dissociate individual neurons. Cytosine arabinoside Sigma was added on the second day of plating neurons to inhibit the replication of non-neuronal cells.

Neuronal cultures were maintained by replacing half of the medium every 5 days. Cells were cultured for 7—15 days before exposure to 20 mm KCl for 5 h.

TTX Sigma at a final concentration of 0.

Applications of STEM (Science, Technology, Engineering and Mathematics - Google Livros

Cultures were exposed to TTX for 3 days. All cells were harvested on the same bglii fermentas for each experiment, and 3C samples were prepared for each group. Chromosome Conformation Capture 3C experiments were performed as bglii fermentas previously 31 with minor modifications.